Evaluation of Oxidative Stress in Serum of Critically Ill Patients by a Commercial Assay and Gas Chromatography–Mass Spectrometry

Cighetti G, Paroni R, Marzorati S, Borotto E, Giudici R, Magnanini G, Iapichino G

Clinical Chemistry ,51(8), 2005

The aim of the present study was to compare a commercial assay (D-ROMs TestTM; Diacron) for assessing oxidative stress in sera of healthy persons and critically ill patients admitted to an intensive care unit (ICU) with an isotope-dilution gas chromatography–mass spectrometry (ID-GC-MS) method, the most specific and sensitive method in this field for measuring F- and T-MDA concentrations.

The assay, which detects serum hydroperoxides as their derivatives (D-ROMs), is based on the reaction of serum with transition metal ions (ferrous sulfate) to form alkoxy and peroxy radicals. The in vitro–formed radicals react with a chromophore compound, generating a radical cation detectable spectrophotometrically at 505 nm; the resulting D-ROMs concentrations are reported in Carratelli units (U.CARR).

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